Apoptosis Reagents and Kits
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 4L
Duolink(R) In Situ Wash Buffers, Fluorescence | 4L
AdipoGen b-AP15
Chemical. CAS 1009817-63-3. Formula C22H17N3O6. MW 419.4. b-AP15 is a specific deubiquitinase DUB inhibitor. It inhibits ubiquitin-specific-processing protease 14 USP14 and ubiquitin c-terminal hydrolase isozyme L5 UCHL5 UCH37, two 19S regulatory particle proteasome-associated DUBs. It exhibits minimal inhibition of UCHL-1, UCHL-3, USP2, USP7, USP and BAP1. Has been described to inhibit also the 20S proteasome with an IC50 comparable to that required for inhibiting the 19S regulatory particles. b-AP15 has anticancer properties and blocks tumor progression in vivo in mice and prevents organ infiltration in mouse models of myeloid leukemia. b-AP15 also renders tumor cells sensitive to TNF-mediated apoptosis by natural killer and T cells. It induces apoptosis, ER stress and autophagy in several cancer cells. b-AP15 has anti-inflammatory properties inhibiting ERK1/2 and JNK phosphorylation, increasing IkappaBalpha levels and inhibiting NF-kappaB p65.
Biotium JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays)
The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation. JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells. In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence (590 nm). The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent (529 nm) monomeric form. This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorometric microplate reader.
Biolegend PE Annexin V Apoptosis Detection Kit with 7-AAD
PE Annexin V Apoptosis Detection Kit with 7-AAD Reactivity: All mammalian species; Apps: FC; Size: 100 tests
Cusabio Technology LLC Rat Caspase 3Casp-3 ELISA Kit
This kit detects CASP3 (Caspase-3) in rat serum plasma and tissue homogenates using a quantitative sandwich ELISA method It offers a detection range of 0 312 ng/mL to 20 ng/mL with a sensitivity of 0 078 ng/mL enabling precise measurement of low-abundance targets The assay requires 50-100 L of sample per test and can be completed within 1-5 hours Results are measured at an absorbance wavelength of 450 nm providing reliable data for apoptosis-related research Designed for flexibility it accommodates multiple biological matrices while maintaining robust performance making it suitable for both routine laboratory analysis and experimental studies requiring accurate quantification of Caspase-3 activity
TRIBIOSCIENCE INC Caspase-1 Fluorometric Assay Kit 100 Tests
Tribioscience Caspase-1 Activity Fluorometric Assay Kit provides a simple and convenient method for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate YVAD-AFC. The free AFC can be quantified using a fluorometer or a fluorescence microtiter plate reader at Ex400/Em505nm. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows the determination of the fold increase in caspase-1 activity.
Abcam Human Procalcitonin Matched Antibody Pair Kit, 5 x 96 Tests
Human Procalcitonin Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Procalcitonin by sandwich ELISA method.
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology Annexin A2 Rabbit pAb
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. Annexin A2 expression has been found to correlate with resistance to treatment against various cancer forms.
Abcam Human DAB2 knockout HeLa cell lysate.
Human DAB2 knockout HeLa cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human SPAST (Spastin) knockout HEK-293T cell lysate.
Human SPAST (Spastin) knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
AdipoGen Raptinal,Chemical. CAS: 1176-09-6
Raptinal, AdipoGen Life Sciences. Chemical. CAS: 1176-09-6. Formula: C28H18O2. MW: 386.4. Rapid apoptosis inducer that initiates intrinsic pathway caspase-dependent apoptosis within minutes in multiple cell lines and in vivo systems. Exerts anticancer activity in vivo, likely through the induction of apoptosis. Induces cell death against various cancer and non-cancerous cell lines with 24 hour IC50 values between 0.7-3.4?M indicating activity across a wide range of cell lines. Activates the intrinsic pathway and requires functional mitochondria for apoptosis induction. Treated cells did not undergo mitochondrial-permeability-transition-pore (MPTP) formation, suggesting a mitochondrial outer membrane permeabilization (MOMP) model of cytochrome c release.
CPC Scientific Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Ala-Asp-CHO 1MG
ONE-LETTER SEQUENCE: Ac-AAVALLPAVLLALLAPVAD-CHOMOLECULAR FORMULA: C88H151N19O22MOLECULAR WEIGHT:1827.3STORAGE CONDITIONS: -20 5CRESEARCH AREA: ApoptosisREFERENCES: N.A. Thornberry, and Y. Lazebnik, Science 281, 1312 (1998); Y-Z Lin, et al., J. Biol. Chem., 271, 5305 (1996); Y-Z Lin, et al., J. Biol. Chem., 270, 14255 (1995)
Abcam Human UBE2C knockout HeLa cell lysate.
Human UBE2C knockout HeLa cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human IFNGR1 knockout HeLa cell lysate.
Human IFNGR1 knockout HeLa cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical QPlexAutoANTIBDY DetectIo 96
An activator of the STING pathway induces secretion of IFN-B in human PBMCs (EC50 130 nM) increases serum levels of Ifn-B Il-6 Tnf and Cxcl1 in wild-type but not Sting-/- mice at 2.5 mg/kg inhibits the cytopathic effect of HCoV-229E in infected MRC-5 cells (EC50 3 nM) decreases the level of SARS-CoV-2 RNA in primary human bronchial airway epithelial cells in an air-liquid interface assay decreases tumor volume and increases survival in a CT26 murine colorectal cancer model at 3 mg/kg this compound is expected to exist in one or both tautomeric forms